| SISpectradynamics INC | Louisville | — | ● ACTIVE |
 Spectra Logic Corporation | Boulder | — | ● ACTIVE |
 Stable Laser Systems INC | Boulder | — | ● ACTIVE |
 Teclis America Corporation | Boulder | — | ● ACTIVE |
 Teraquant Corporation | Boulder | — | ● ACTIVE |
 Umorpha Systems LLC | Boulder | — | ● ACTIVE |
 Vaisala INC. | Louisville | — | ● ACTIVE |
 Vitawave Tech, INC | Boulder | — | ● ACTIVE |
 Xeed, LLC | Boulder | — | ● ACTIVE |
 Xetawave LLC | Louisville | — | ● ACTIVE |
| DSDieterich Standard INC | Boulder | — | ◇ UNVERIFIED |
 Nanoplus America, INC. | Boulder | — | ◐ LIKELY ACTIVE |
 Pixcell Medical, INC | Longmont | — | ◐ LIKELY ACTIVE |
| MFMicrosemi Frequency and Time CORP | Boulder | — | ◐ LIKELY ACTIVE |
| VBVaisala BOU | Louisville | 100–499 | ◐ LIKELY ACTIVE |
| APAdvanced Probing Systems | Louisville | — | ◇ UNVERIFIED |
| BEBoulder Elec Motor CO | Boulder | — | ◇ UNVERIFIED |
| CCCircle Circuits | Boulder | — | ◇ UNVERIFIED |
| CICircuit Images, INC | Boulder | — | ◇ UNVERIFIED |
| CWCircuits West INC | Longmont | — | ◇ UNVERIFIED |
| DFDesign Fabricators INC | Boulder | — | ◇ UNVERIFIED |
| DIDotronix INC | Boulder | — | ◇ UNVERIFIED |
| EMEngineering Measurement CO. | Longmont | — | ◇ UNVERIFIED |
| SCSae Circuits Colorado INC | Boulder | — | ◇ UNVERIFIED |
| SCSae Circuits Colorado INC | Boulder | — | ◇ UNVERIFIED |
 Seagate Technology LLC | Longmont | — | ◇ UNVERIFIED |
| SISheldahl INC | Longmont | — | ◇ UNVERIFIED |
| STStorage Technology CORP Clover Basin Dr | Longmont | — | ◇ UNVERIFIED |
| SMSun Microsystems INC | Louisville | — | ◇ UNVERIFIED |
 Blue Canyon Technologies - Crescent, LLC | Lafayette | 100–499 | ◐ LIKELY ACTIVE |
| EIEquinox Interscience, INC. | Nederland | — | ▲ REPORTED CLOSED |
| FTFlari Tech INC | Boulder | — | ◐ LIKELY ACTIVE |
| LLongmont | Longmont | 20–99 | ◐ LIKELY ACTIVE |
| MCMedtronic Covidien LP | Boulder | 500+ | ◐ LIKELY ACTIVE |
| MFMicrosemi FTS Inc (A Microchip Co) | Boulder | 20–99 | ◐ LIKELY ACTIVE |
| OIOxford Ionics North America INC | Boulder | — | ◐ LIKELY ACTIVE |
| PTPage Technologies, INC. | Boulder | — | ◐ LIKELY ACTIVE |
| PMParticle Measuring Systems, INC | Niwot | — | ▲ REPORTED CLOSED |
| QLQSC LLC - Boulder | Boulder | 20–99 | ◐ LIKELY ACTIVE |
| SLSandisk Longmont | Longmont | 20–99 | ◐ LIKELY ACTIVE |
| BSBae Systems Space & Mission Systems Boulder | Boulder | — | ◇ UNVERIFIED |
| BABall Aerospace & Technologies CORP | Lafayette | — | ◇ UNVERIFIED |
| CWCircuits West LLC | Longmont | — | ◇ UNVERIFIED |
| CPConner Peripherals | Longmont | — | ◇ UNVERIFIED |
| GLGoomwave LLC | Erie | — | ◇ UNVERIFIED |
| LTLarcan Tcc INC | Lousiville | — | ◇ UNVERIFIED |
| MRMisty Robotics | Boulder | — | ◇ UNVERIFIED |
| MQMke - Quantum Corpation (was Periphera | Louisville | — | ◇ UNVERIFIED |
| OOhmeda | Louisville | — | ◇ UNVERIFIED |
| ZSZonit Structured Solutions LLC | Boulder | — | ◇ UNVERIFIED |
