| CColorbiotics | Ames | IA | Chemical Manufacturing325130 | 20-99 | ◐ LIKELY ACTIVE |
| 3M3m (minnesota Mining & Mfg Co) - Ames | Ames | IA | Nonmetallic Mineral Product Manufacturing327910 | — | ◐ LIKELY ACTIVE |
| BABarilla America INC | Ames | IA | Food Manufacturing311823 | 100-499 | ◐ LIKELY ACTIVE |
| CICentral Iowa Ready Mix-Ames | Ames | IA | Nonmetallic Mineral Product Manufacturing327320 | 1-19 | ◐ LIKELY ACTIVE |
| VAVermeer Ames | Ames | IA | Machinery Manufacturing333120 | 20-99 | ◐ LIKELY ACTIVE |
| SMSargeant Metal Fabricating | Ames | IA | Textile Mills313320 | — | ◐ LIKELY ACTIVE |
| SCSigler Companies | Ames | IA | Printing and Related Support Activities323110 | — | ◐ LIKELY ACTIVE |
| ABAmes Branch | Ames | IA | Beverage and Tobacco Product Manufacturing312111 | 20-99 | ◐ LIKELY ACTIVE |
| ARAya Ranch, L.c. | Ames | IA | Beverage and Tobacco Product Manufacturing312130 | — | ◐ LIKELY ACTIVE |
| BABASF Agricultural Solutions US LLC | Ames | IA | Chemical Manufacturing325130 | 20-99 | ◐ LIKELY ACTIVE |
| BLBiova LLC | Ames | IA | Food Manufacturing311999 | 1-19 | ◐ LIKELY ACTIVE |
| CPCoherent Photon Imaging, LLC | Ames | IA | Computer and Electronic Product Manufacturing334516 | — | ◐ LIKELY ACTIVE |
| DDSM-Firmenich | Ames | IA | Food Manufacturing311111 | 20-99 | ◐ LIKELY ACTIVE |
| ISIowa State University Meat Laboratory | Ames | IA | Food Manufacturing311611 | — | ◐ LIKELY ACTIVE |
| ISIowa State University Winery | Ames | IA | Beverage and Tobacco Product Manufacturing312130 | — | ◐ LIKELY ACTIVE |
| MAMerck Ames, IA Facility | Ames | IA | Chemical Manufacturing325414 | 20-99 | ◐ LIKELY ACTIVE |
| STShivvers Technology & Innovation Group | Ames | IA | Machinery Manufacturing333111 | 1-19 | ◐ LIKELY ACTIVE |
| SASukup Ames Satellite Office | Ames | IA | Machinery Manufacturing333111 | 20-99 | ◐ LIKELY ACTIVE |
| TNTech Network Supply LLC | Ames | IA | Computer and Electronic Product Manufacturing334112 | — | ◐ LIKELY ACTIVE |