| GMGiese Manufacturing | Dubuque | IA | Fabricated Metal Product Manufacturing332322 | 20-99 | ◐ LIKELY ACTIVE |
| JDJohn Deere Dubuque Works | Dubuque | IA | Machinery Manufacturing333111 | 500+ | ◐ LIKELY ACTIVE |
| MBMorrison Brothers Company | Dubuque | IA | Primary Metal Manufacturing331524 | 1-19 | ◐ LIKELY ACTIVE |
| PFPrairie Farms-dubuque | Dubuque | IA | Food Manufacturing311511 | 100-499 | ◐ LIKELY ACTIVE |
Rie Coatings INC | Dubuque | IA | Fabricated Metal Product Manufacturing332812 | 1-19 | ◐ LIKELY ACTIVE |
| ADApc Dubuque | Dubuque | IA | Food Manufacturing311119 | 20-99 | ◐ LIKELY ACTIVE |
| ECEdwards Cast Stone Company | Dubuque | IA | Nonmetallic Mineral Product Manufacturing327390 | 20-99 | ◐ LIKELY ACTIVE |
| FHFlint Hills Resources Pine Bend LLC - Dubuque Terminal | Dubuque | IA | Petroleum and Coal Products Manufacturing324121 | — | ◐ LIKELY ACTIVE |
Banvue Point International, INC | Dubuque | IA | Computer and Electronic Product Manufacturing334516 | — | ◐ LIKELY ACTIVE |
| DIDubuque Ia Bakery | Dubuque | IA | Food Manufacturing311812 | 100-499 | ◐ LIKELY ACTIVE |
| EWEagel Window & Door INC. | Dubuque | IA | Fabricated Metal Product Manufacturing332321 | — | ◐ LIKELY ACTIVE |
| EWEagle Window & Door INC. | Dubuque | IA | Wood Product Manufacturing321911 | — | ◐ LIKELY ACTIVE |
| FUF.h. Uelner Precision Tools and Dies, INC. | Dubuque | IA | Machinery Manufacturing333514 | 20-99 | ◐ LIKELY ACTIVE |
| RDRousselot Dubuque INC. | Dubuque | IA | Chemical Manufacturing325411 | 20-99 | ◐ LIKELY ACTIVE |
| IFInnocor Foam Technologies (formerly Advanced Urethane Technologies) | Dubuque | IA | Chemical Manufacturing325211 | — | ◐ LIKELY ACTIVE |
| AKAinley Kennels & Fabrication, INC. | Dubuque | IA | Fabricated Metal Product Manufacturing332322 | — | ◐ LIKELY ACTIVE |
| AWAndersen Windows and Doors - Dubuque, IA. | Dubuque | IA | Fabricated Metal Product Manufacturing332321 | 500+ | ◐ LIKELY ACTIVE |
| BHBarrel Head | Dubuque | IA | Beverage and Tobacco Product Manufacturing312130 | — | ◐ LIKELY ACTIVE |
| CCCrown Cork & Seal USA, Inc. - Dubuque Plant | Dubuque | IA | Fabricated Metal Product Manufacturing332431 | 20-99 | ◐ LIKELY ACTIVE |
| DDubuque | Dubuque | IA | Fabricated Metal Product Manufacturing332431 | 20-99 | ◐ LIKELY ACTIVE |
| DBDubuque Branch | Dubuque | IA | Beverage and Tobacco Product Manufacturing312111 | 20-99 | ◐ LIKELY ACTIVE |
| DHDubuque Heritage Winery | Dubuque | IA | Beverage and Tobacco Product Manufacturing312130 | — | ◐ LIKELY ACTIVE |
| DPDubuque Pet Food | Dubuque | IA | Food Manufacturing311111 | 100-499 | ◐ LIKELY ACTIVE |
| DSDubuque Screw Products | Dubuque | IA | Fabricated Metal Product Manufacturing332710 | 20-99 | ◐ LIKELY ACTIVE |
| DSDubuque Steel Products | Dubuque | IA | Fabricated Metal Product Manufacturing332311 | 20-99 | ◐ LIKELY ACTIVE |
| FIFXI, Inc. - Dubuque | Dubuque | IA | Plastics and Rubber Products Manufacturing326150 | 1-19 | ◐ LIKELY ACTIVE |
| GWGrampas Wine | Dubuque | IA | Beverage and Tobacco Product Manufacturing312130 | — | ◐ LIKELY ACTIVE |
| HVHilltop Vineyard and Winery | Dubuque | IA | Beverage and Tobacco Product Manufacturing312130 | — | ◐ LIKELY ACTIVE |
| MBMorrison Bros. Mfg. | Dubuque | IA | Fabricated Metal Product Manufacturing332911 | 20-99 | ◐ LIKELY ACTIVE |
| PDParadise Distilling Company, LLC | Dubuque | IA | Beverage and Tobacco Product Manufacturing312140 | — | ◐ LIKELY ACTIVE |
| SCStone Cliff Winery | Dubuque | IA | Beverage and Tobacco Product Manufacturing312130 | — | ◐ LIKELY ACTIVE |
| SRSunset Ridge Winery | Dubuque | IA | Beverage and Tobacco Product Manufacturing312130 | — | ◐ LIKELY ACTIVE |
| PIPaja INC. | Dubuque | IA | Printing and Related Support Activities323111 | — | ◐ LIKELY ACTIVE |