| SBS & B Tool LLC | Middleton | ID | Fabricated Metal Product Manufacturing332710 | — | ◐ LIKELY ACTIVE |
| SISeawright, INC. | Middleton | FL | Transportation Equipment Manufacturing336611 | — | ◐ LIKELY ACTIVE |
| SSSunbelt Solomon Services-CTMT | Middleton | CT | Electrical Equipment, Appliance & Component Manufacturing335311 | 20-99 | ◐ LIKELY ACTIVE |
| SNSun Nuclear CORP. | Middleton | WI | Miscellaneous Manufacturing339112 | — | ◐ LIKELY ACTIVE |
| TETK Elevator Middleton MFG | Middleton | TN | Machinery Manufacturing333921 | 500+ | ◐ LIKELY ACTIVE |
| UPUW Provision Company, Inc. | Middleton | WI | Food Manufacturing311612 | — | ◐ LIKELY ACTIVE |
| DDDeath's Door Distillery, LLC | Middleton | WI | Beverage and Tobacco Product Manufacturing312140 | — | ◇ UNVERIFIED |
| ELEmw LLC | Middleton | MA | Fabricated Metal Product Manufacturing332710 | — | ◇ UNVERIFIED |
| ALAesthreadics LLC | Middleton | ID | Leather and Allied Product Manufacturing316990 | — | ◇ UNVERIFIED |
| APAg Precision Formulators | Middleton | WI | Food Manufacturing311999 | — | ◇ UNVERIFIED |
| ALArmset LLC | Middleton | MA | Fabricated Metal Product Manufacturing332216 | — | ◇ UNVERIFIED |
| APAstro Pak LLC | Middleton | WI | Fabricated Metal Product Manufacturing332813 | — | ◇ UNVERIFIED |
| ACAutomated Components, INC. | Middleton | WI | Computer and Electronic Product Manufacturing334512 | — | ◇ UNVERIFIED |
| BFBen Franklin Print Co., Inc. | Middleton | MA | Printing and Related Support Activities323111 | — | ◇ UNVERIFIED |
| BFBen Franklin Print CO INC | Middleton | MA | Printing and Related Support Activities323113 | — | ◇ UNVERIFIED |
| BBBloom Bindery, LLC | Middleton | WI | Food Manufacturing311811 | — | ◇ UNVERIFIED |
| BIBruker INC | Middleton | WI | Computer and Electronic Product Manufacturing334516 | — | ◇ UNVERIFIED |
| BNBruker Nano, INC. | Middleton | WI | Computer and Electronic Product Manufacturing334516 | — | ◇ UNVERIFIED |
| DLDrs LTD | Middleton | WI | Petroleum and Coal Products Manufacturing324121 | — | ◇ UNVERIFIED |
| ELEpac LLC | Middleton | WI | Printing and Related Support Activities323111 | — | ◇ UNVERIFIED |
| HSHazy Skies Designs, LLC | Middleton | ID | Printing and Related Support Activities323111 | — | ◇ UNVERIFIED |
| LILycon INC - Middleton - University Ave | Middleton | WI | Nonmetallic Mineral Product Manufacturing327320 | — | ◇ UNVERIFIED |
| MPMcNic Properties LLC | Middleton | ID | Fabricated Metal Product Manufacturing332999 | — | ◇ UNVERIFIED |
| MFMiddleton Farmers Cooperative Company | Middleton | WI | Food Manufacturing311119 | — | ◇ UNVERIFIED |
| MMMiddleton Machining & Welding, INC. | Middleton | WI | Fabricated Metal Product Manufacturing332710 | — | ◇ UNVERIFIED |
| MBMiggy's Bakes, LLC | Middleton | WI | Food Manufacturing311811 | — | ◇ UNVERIFIED |
| MSMK Services Corp. | Middleton | MA | Fabricated Metal Product Manufacturing332710 | — | ◇ UNVERIFIED |
| NINastar, INC | Middleton | WI | Printing and Related Support Activities323119 | — | ◇ UNVERIFIED |
| NSNewcastle Systems, INC. | Middleton | MA | Furniture and Related Product Manufacturing337124 | — | ◇ UNVERIFIED |
| PTPhoenix Technique LLC | Middleton | WI | Transportation Equipment Manufacturing336310 | — | ◇ UNVERIFIED |
| PMPrefinished Millwork CORP | Middleton | WI | Wood Product Manufacturing321910 | — | ◇ UNVERIFIED |
| RFRichardson's Farms, INC. | Middleton | MA | Food Manufacturing311520 | — | ◇ UNVERIFIED |
| RCRule Custom Fabrication, LLC | Middleton | ID | Fabricated Metal Product Manufacturing332999 | — | ◇ UNVERIFIED |
| SSSierra Space | Middleton | WI | Transportation Equipment Manufacturing336415 | — | ◇ UNVERIFIED |
| TEThyssenkrupp Elevator Manufacturing, INC. | Middleton | TN | Machinery Manufacturing333921 | — | ◇ UNVERIFIED |
| VCViking Cues, LLC | Middleton | WI | Miscellaneous Manufacturing339920 | — | ◇ UNVERIFIED |
| WMWorcester Marble & Granite INC. | Middleton | MA | Nonmetallic Mineral Product Manufacturing327991 | — | ◇ UNVERIFIED |
| YFYankee Fireplace Grill & Patio | Middleton | MA | Machinery Manufacturing333414 | — | ◇ UNVERIFIED |
