| AEAberdeen Energy LLC | Mina | 20–99 | ● ACTIVE |
A-ox Welding Supply, LLC | Sioux Falls | — | ● ACTIVE |
Dakota Ethanol LLC | Wentworth | 20–99 | ● ACTIVE |
Glacial Lakes Energy LLC | Watertown | 20–99 | ● ACTIVE |
Henkel US Operations Corporation | Brandon | — | ● ACTIVE |
| NENugen Energy LLC | Marion | 20–99 | ● ACTIVE |
| PBPoet Bioprocessing Hudson Co2 Plant Addition | Hudson | 20–99 | ● ACTIVE |
| PBPoet Biorefining - Groton | Groton | 20–99 | ● ACTIVE |
| PRPoet Research Center | Scotland | 20–99 | ● ACTIVE |
| RRRed River Energy LLC | Rosholt | 20–99 | ● ACTIVE |
| RERingneck Energy, LLC | Onida | 20–99 | ● ACTIVE |
Amtec CORP | Clear Lake | — | ● ACTIVE |
| RERedfield Energy, LLC | Redfield | 20–99 | ● ACTIVE |
A & B Welding Supply LLC | Rapid City | — | ● ACTIVE |
| ATAntiradical Therapeutics LLC | Sioux Falls | — | ● ACTIVE |
Armourer's Choice, LLC | Sioux Falls | — | ● ACTIVE |
Caw Industries, INC. | Rapid City | — | ● ACTIVE |
Cytion LLC | Sioux Falls | — | ● ACTIVE |
| HCHub City Energy LLC | Aberdeen | 20–99 | ● ACTIVE |
| HEHuron Energy LLC | Huron | 20–99 | ● ACTIVE |
Van Diest Supply Company | Pierre | — | ● ACTIVE |
Vst LLC | Brookings | — | ● ACTIVE |
Zanfel Laboratories, INC | Dakota Dunes | — | ● ACTIVE |
| PBPoet Biorefining Big Stone | Big Stone City | 20–99 | ◐ LIKELY ACTIVE |
| BFBenchmark Foam, INC. | Watertown | 20–99 | ◐ LIKELY ACTIVE |
| OTOtter Tail Power Company | Big Stone City | — | ◐ LIKELY ACTIVE |
| PEPrairie Ethanol, LLC | Mitchell | — | ◐ LIKELY ACTIVE |
| TOTech Ord, a Division of Amtec Corporation | Clear Lake | 100–499 | ◐ LIKELY ACTIVE |
| VRValero Renewable Fuels Company | Aurora | — | ◐ LIKELY ACTIVE |
| AHAbe Heartland, LLC Huron Facility | Huron | — | ◇ UNVERIFIED |
| CEChemring Energetic Devices | Clear Lake | — | ◇ UNVERIFIED |
| WCWalling Chemical Company | Sioux Falls | — | ◇ UNVERIFIED |
| HCHub City Energy LLC | Aberdeen | — | ◐ LIKELY ACTIVE |
| HEHuron Energy Llc, Dba Glacial Lakes Energy LLC | Huron | — | ◐ LIKELY ACTIVE |
| ISIdic Specialty Drilling Chemicals LLC | Belle Fourche | — | ◐ LIKELY ACTIVE |
| SESiouxland Energy Cooperative | Hudson | — | ◐ LIKELY ACTIVE |
| AAfco | Brandon | — | ◇ UNVERIFIED |
| TAToshiba America Information Systems Incorporated Toner Products Division | Mitchell | — | ◇ UNVERIFIED |
| ACAgland Coop | Parkston | — | ◐ LIKELY ACTIVE |
| DPDak Pak LLC | Woonsocket | 20–99 | ◐ LIKELY ACTIVE |
| DRD & R Propane, INC. | Eagle Butte | — | ◐ LIKELY ACTIVE |
| EPETRIA Products USA INC. | Mitchell | 20–99 | ◐ LIKELY ACTIVE |
| FSFinishWorks (Tea, SD) | Tea | 1–19 | ◐ LIKELY ACTIVE |
| FCFull Circle Ag | Britton | — | ◐ LIKELY ACTIVE |
| MMedgene | Brookings | 100–499 | ◐ LIKELY ACTIVE |
| PBPOET Bioprocessing- Chancellor | Chancellor | 20–99 | ◐ LIKELY ACTIVE |
| PBPOET Bioprocessing Mitchell | Mitchell | 20–99 | ◐ LIKELY ACTIVE |
| RERing-neck Energy & Feed, LLC | Onida | — | ◐ LIKELY ACTIVE |
| SLSenproco, LLC | Sioux Falls | 20–99 | ◐ LIKELY ACTIVE |
| VRValero Renewable Fuels Company, LLC dba Valero Aurora Plant | Aurora | 20–99 | ◐ LIKELY ACTIVE |