| BCBurke Corporation | Nevada, IA | Food Manufacturing311612 | 500+ | ● ACTIVE |
Composite Technologies LLC | Boone, IA | Plastics and Rubber Products Manufacturing326199 | — | ● ACTIVE |
| HCHach Company | Ames, IA | Chemical Manufacturing325188 | 100–499 | ● ACTIVE |
| LELincolnway Energy LLC | Nevada, IA | Chemical Manufacturing325193 | 20–99 | ● ACTIVE |
Agcertain Industries INC | Ames, IA | Food Manufacturing311225 | 1–19 | ● ACTIVE |
Powerfilm, INC. | Ames, IA | Computer and Electronic Product Manufacturing334413 | 20–99 | ● ACTIVE |
Reg Synthetic Fuels, LLC | Ames, IA | Petroleum and Coal Products Manufacturing324199 | — | ● ACTIVE |
Technical Services Incorporated | Ames, IA | Fabricated Metal Product Manufacturing332116 | — | ● ACTIVE |
Ag Leader Technology, INC | Ames, IA | Computer and Electronic Product Manufacturing334419 | — | ● ACTIVE |
Ames Engineering LLC | Ames, IA | Computer and Electronic Product Manufacturing334519 | — | ● ACTIVE |
Gary W Clem INC | Nevada, IA | Machinery Manufacturing333111 | — | ● ACTIVE |
Genvax Technologies INC | Ames, IA | Chemical Manufacturing325414 | — | ● ACTIVE |
Hanger Prosthetics & Orthotics, INC. | Ames, IA | Miscellaneous Manufacturing339113 | — | ● ACTIVE |
Novascan Technologies, INC. | Boone, IA | Computer and Electronic Product Manufacturing334419 | — | ● ACTIVE |
Soylei Innovations, LLC | Ames, IA | Chemical Manufacturing325199 | — | ● ACTIVE |
Strength Technologies LLC | Ames, IA | Miscellaneous Manufacturing339920 | — | ● ACTIVE |
United Equipment Accessories INC | Ames, IA | Fabricated Metal Product Manufacturing332999 | — | ● ACTIVE |
Variety Vault LLC | Ames, IA | Plastics and Rubber Products Manufacturing326199 | — | ● ACTIVE |
W and G Marketing Company, INC. | Ames, IA | Food Manufacturing311612 | — | ● ACTIVE |
| CColorbiotics | Ames, IA | Chemical Manufacturing325130 | 20–99 | ◐ LIKELY ACTIVE |
| 3M3m (minnesota Mining & Mfg Co) - Ames | Ames, IA | Nonmetallic Mineral Product Manufacturing327910 | — | ◐ LIKELY ACTIVE |
| BABarilla America INC | Ames, IA | Food Manufacturing311823 | 100–499 | ◐ LIKELY ACTIVE |
| APAmerican Packaging Corporation | Story City, IA | Printing and Related Support Activities323111 | — | ◐ LIKELY ACTIVE |
| BUBecker Underwood INC | Ames, IA | Chemical Manufacturing325130 | — | ◇ UNVERIFIED |
| BQBesser Quinn Machine & Foundry | Boone, IA | Primary Metal Manufacturing331511 | — | ◇ UNVERIFIED |
| DNDsm Nutritional Products INC | Ames, IA | Food Manufacturing311119 | — | ◇ UNVERIFIED |
| SISauer-danfoss INC | Ames, IA | Machinery Manufacturing333611 | — | ◇ UNVERIFIED |
| CMContinental Manufacturing Chemist INC | Madrid, IA | Chemical Manufacturing325611 | — | ◇ UNVERIFIED |
| BIBuckaroos, INC. | Nevada, IA | Fabricated Metal Product Manufacturing332999 | 20–99 | ◐ LIKELY ACTIVE |
| CICentral Iowa Ready Mix-Ames | Ames, IA | Nonmetallic Mineral Product Manufacturing327320 | 1–19 | ◐ LIKELY ACTIVE |
| CMContinental Manufacturing Chemist, Inc. | Huxley, IA | Chemical Manufacturing325611 | — | ◐ LIKELY ACTIVE |
| DCDupont Cellulosic Ethanol | Nevada, IA | Chemical Manufacturing325193 | — | ◐ LIKELY ACTIVE |
| RPRecord Printing Company, Inc. of Story City | Story City, IA | Printing and Related Support Activities323111 | 20–99 | ◐ LIKELY ACTIVE |
| VAVermeer Ames | Ames, IA | Machinery Manufacturing333120 | 20–99 | ◐ LIKELY ACTIVE |
| SMSargeant Metal Fabricating | Ames, IA | Textile Mills313320 | — | ◐ LIKELY ACTIVE |
| SCSigler Companies | Ames, IA | Printing and Related Support Activities323110 | — | ◐ LIKELY ACTIVE |
| AIAlmaco INC | Nevada, IA | Machinery Manufacturing333111 | — | ◇ UNVERIFIED |
| CICentral Iowa Ready Mix | Boone, IA | Nonmetallic Mineral Product Manufacturing327320 | — | ◇ UNVERIFIED |
| HCHome City Ice | Ames, IA | Beverage and Tobacco Product Manufacturing312113 | — | ◇ UNVERIFIED |
| IPIndustrial Plating CO | Ames, IA | Fabricated Metal Product Manufacturing332813 | — | ◇ UNVERIFIED |
| ILIspt LLC | Story City, IA | Fabricated Metal Product Manufacturing332710 | — | ◇ UNVERIFIED |
| KGKemx Global | Boone, IA | Food Manufacturing311225 | — | ◇ UNVERIFIED |
| KCKiliper Corporation | Ames, IA | Printing and Related Support Activities323111 | — | ◇ UNVERIFIED |
| MIManatt's INC - Huxley | Huxley, IA | Nonmetallic Mineral Product Manufacturing327320 | — | ◇ UNVERIFIED |
| MHM H Eby INC | Story City, IA | Machinery Manufacturing333111 | — | ◇ UNVERIFIED |
| PCPella Corporation - Story City Division | Story City, IA | Wood Product Manufacturing321911 | — | ◇ UNVERIFIED |
| PEPriority Envelope INC | Nevada, IA | Paper Manufacturing322232 | — | ◇ UNVERIFIED |
| PHProliant Health & Biologicals | Boone, IA | Food Manufacturing311613 | — | ◇ UNVERIFIED |
| ABAgCertain Boone, LLC | Boone, IA | Food Manufacturing311225 | 20–99 | ◐ LIKELY ACTIVE |
| AAmes | Ames, IA | Nonmetallic Mineral Product Manufacturing327910 | 100–499 | ◐ LIKELY ACTIVE |