| MCMiddlesboro Coca-cola Bottling Works, INC. | Middlesboro, KY | Beverage and Tobacco Product Manufacturing312111 | 100–499 | ● ACTIVE |
| SPSmithfield Packaged Meats CORP - Middlesboro | Middlesboro, KY | Food Manufacturing311612 | — | ● ACTIVE |
 Southeastern Kentucky Rehabilitation Industries, INC | Pineville, KY | Apparel Manufacturing315990 | — | ● ACTIVE |
| BCBell Concrete Industries INC | Middlesboro, KY | Nonmetallic Mineral Product Manufacturing327332 | — | ◐ LIKELY ACTIVE |
| DCDura-line CORP | Middlesboro, KY | Plastics and Rubber Products Manufacturing326121 | — | ◐ LIKELY ACTIVE |
| HCHinkle Contracting CO LLC - Middlesboro Asphalt Plant | Middlesboro, KY | Petroleum and Coal Products Manufacturing324121 | — | ◐ LIKELY ACTIVE |
| JRJ R Hoe & Sons INC | Middlesboro, KY | Primary Metal Manufacturing331511 | — | ◐ LIKELY ACTIVE |
| MCMiddlesboro Concrete Plant | Middlesboro, KY | Nonmetallic Mineral Product Manufacturing327320 | — | ◐ LIKELY ACTIVE |
| MTMountain Tarp | Middlesboro, KY | Textile Product Mills314910 | — | ◐ LIKELY ACTIVE |
| BCBell Concrete Industries | Middlesboro, KY | Nonmetallic Mineral Product Manufacturing327320 | — | ◇ UNVERIFIED |
| BCBell Concrete Industries INC | Middlesboro, KY | Nonmetallic Mineral Product Manufacturing327332 | — | ◇ UNVERIFIED |
| CPConcrete Products INC | Middlesboro, KY | Nonmetallic Mineral Product Manufacturing327320 | — | ◇ UNVERIFIED |
| JSJ & S Logging & Lumber | Arjay, KY | Wood Product Manufacturing321113 | — | ◇ UNVERIFIED |
| TCTri-state Concrete | Middlesboro, KY | Nonmetallic Mineral Product Manufacturing327320 | — | ◇ UNVERIFIED |
 Wastequip Manufacturing CO LLC | Middlesboro, KY | Fabricated Metal Product Manufacturing332117 | — | ◇ UNVERIFIED |
| AFArveybell Farm Co. | Middlesboro, KY | Food Manufacturing311612 | — | ◐ LIKELY ACTIVE |
| BDBlue Diamond Industries Middlesboro | Middlesboro, KY | Plastics and Rubber Products Manufacturing326122 | 100–499 | ◐ LIKELY ACTIVE |
| SMSmithfield - Middlesboro Packaged | Middlesboro, KY | Food Manufacturing311612 | 500+ | ◐ LIKELY ACTIVE |
| CECombs Equipment Group LLC | Middlesboro, KY | Fabricated Metal Product Manufacturing332813 | — | ◇ UNVERIFIED |
| CPConcrete Products INC | Middlesboro, KY | Nonmetallic Mineral Product Manufacturing327320 | — | ◇ UNVERIFIED |
| NENewell Enterprises | Pineville, KY | Nonmetallic Mineral Product Manufacturing327320 | — | ◇ UNVERIFIED |
| SFSmithfield Farmland CORP | Middlesboro, KY | Food Manufacturing311611 | — | ◇ UNVERIFIED |
