| MCMiddlesboro Coca-cola Bottling Works, INC. | Middlesboro | Beverage and Tobacco Product Manufacturing | 312111 | 100–499 | ● ACTIVE |
| SPSmithfield Packaged Meats CORP - Middlesboro | Middlesboro | Food Manufacturing | 311612 | — | ● ACTIVE |
 Southeastern Kentucky Rehabilitation Industries, INC | Pineville | Apparel Manufacturing | 315990 | — | ● ACTIVE |
| BCBell Concrete Industries INC | Middlesboro | Nonmetallic Mineral Product Manufacturing | 327332 | — | ◐ LIKELY ACTIVE |
| DCDura-line CORP | Middlesboro | Plastics and Rubber Products Manufacturing | 326121 | — | ◐ LIKELY ACTIVE |
| HCHinkle Contracting CO LLC - Middlesboro Asphalt Plant | Middlesboro | Petroleum and Coal Products Manufacturing | 324121 | — | ◐ LIKELY ACTIVE |
| JRJ R Hoe & Sons INC | Middlesboro | Primary Metal Manufacturing | 331511 | — | ◐ LIKELY ACTIVE |
| MCMiddlesboro Concrete Plant | Middlesboro | Nonmetallic Mineral Product Manufacturing | 327320 | — | ◐ LIKELY ACTIVE |
| MTMountain Tarp | Middlesboro | Textile Product Mills | 314910 | — | ◐ LIKELY ACTIVE |
| BCBell Concrete Industries | Middlesboro | Nonmetallic Mineral Product Manufacturing | 327320 | — | ◇ UNVERIFIED |
| BCBell Concrete Industries INC | Middlesboro | Nonmetallic Mineral Product Manufacturing | 327332 | — | ◇ UNVERIFIED |
| CPConcrete Products INC | Middlesboro | Nonmetallic Mineral Product Manufacturing | 327320 | — | ◇ UNVERIFIED |
| JSJ & S Logging & Lumber | Arjay | Wood Product Manufacturing | 321113 | — | ◇ UNVERIFIED |
| TCTri-state Concrete | Middlesboro | Nonmetallic Mineral Product Manufacturing | 327320 | — | ◇ UNVERIFIED |
 Wastequip Manufacturing CO LLC | Middlesboro | Fabricated Metal Product Manufacturing | 332117 | — | ◇ UNVERIFIED |
| AFArveybell Farm Co. | Middlesboro | Food Manufacturing | 311612 | — | ◐ LIKELY ACTIVE |
| BDBlue Diamond Industries Middlesboro | Middlesboro | Plastics and Rubber Products Manufacturing | 326122 | 100–499 | ◐ LIKELY ACTIVE |
| SMSmithfield - Middlesboro Packaged | Middlesboro | Food Manufacturing | 311612 | 500+ | ◐ LIKELY ACTIVE |
| CECombs Equipment Group LLC | Middlesboro | Fabricated Metal Product Manufacturing | 332813 | — | ◇ UNVERIFIED |
| CPConcrete Products INC | Middlesboro | Nonmetallic Mineral Product Manufacturing | 327320 | — | ◇ UNVERIFIED |
| NENewell Enterprises | Pineville | Nonmetallic Mineral Product Manufacturing | 327320 | — | ◇ UNVERIFIED |
| SFSmithfield Farmland CORP | Middlesboro | Food Manufacturing | 311611 | — | ◇ UNVERIFIED |
